DNA directed RNA polymerase II A,DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit,DNA-directed RNA polymerase II subunit A,DNA-directed RNA polymerase II subunit RPB1,DNA-directed RNA polymerase III largest subunit,hRPB220,hsRPB1,POLR2,Polr2a,POLRA,Polymerase (RNA) II (DNA directed) polypeptide A 220 kDa,Polymerase (RNA) II (DNA directed) polypeptide A,RNA polymerase II subunit B1,RNA-directed RNA polymerase II subunit RPB1,RPB1,RPB1,RPBh1,RpIILS,RPO2,RPOL2
纯化方法
Affinity purification
免疫原
Synthesized peptide derived from human Rpb1 around the non-phosphorylation site of Ser1619.
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:10000
限制
仅限研究用
浓度
1 mg/mL
缓冲液
PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol, pH 7.4
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Store at -20°C. Avoid freeze / thaw cycles.
抗原
POLR2A/RPB1 (POLR2A)
(RNA Polymerase II Subunit RPB1 (POLR2A))
别名
Rpb1
背景
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.