EIF5A 抗体 (Internal Region)
Quick Overview for EIF5A 抗体 (Internal Region) (ABIN571047)
抗原
See all EIF5A 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
-
-
抗原表位
- Internal Region
-
原理
- eIF5A
-
序列
- RPPHRASFLK RLESK
-
特异性
- Reported variants NP_001137233.1, NP_001961.1 and NP_001137234.1 represent identical protein:
-
交叉反应
- 人
-
纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
免疫原
- Peptide with sequence C-RPPHRASFLKRLESK, from the internal region (near N Terminus) of the protein sequence according to NP_001137232.1.
-
亚型
- IgG
-
-
-
-
应用备注
-
Immunohistochemistry: Paraffin embedded Human Spleen, Tonsil and Vessel. Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 20 kDa band observed in MOLT4 lysates (calculated MW of 20.2 kDa according to NP_001137232.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human, PMID: 27414022
Peptide ELISA: antibody detection limit dilution 1:128000.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.5 mg/mL
-
缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Minimize freezing and thawing.
-
储存条件
- -20 °C
-
储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
-
- EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))
-
别名
- EIF5A
-
背景
- EIF5A, eukaryotic translation initiation factor 5A, EIF-5A, EIF5A1, MGC104255, MGC99547, eIF5AI, OTTHUMP00000128384, uORF A
-
基因ID
- 1984
-
NCBI登录号
- NP_001137232
-
途径
- Regulation of Muscle Cell Differentiation
抗原
-