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EIF5A 抗体 (Internal Region)

The 山羊 多克隆 anti-EIF5A antibody (ABIN571047) specifically detects EIF5A in WB, IHC 和 ELISA. The antibody is reactive with 人 samples.
产品编号 ABIN571047
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for EIF5A 抗体 (Internal Region) (ABIN571047)

抗原

See all EIF5A 抗体
EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This EIF5A antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    eIF5A

    序列

    RPPHRASFLK RLESK

    特异性

    Reported variants NP_001137233.1, NP_001961.1 and NP_001137234.1 represent identical protein:

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-RPPHRASFLKRLESK, from the internal region (near N Terminus) of the protein sequence according to NP_001137232.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Spleen, Tonsil and Vessel. Recommended concentration: 3.75 μg/mL.

    Western Blot: Approx 20 kDa band observed in MOLT4 lysates (calculated MW of 20.2 kDa according to NP_001137232.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human, PMID: 27414022

    Peptide ELISA: antibody detection limit dilution 1:128000.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

    别名

    EIF5A

    背景

    EIF5A, eukaryotic translation initiation factor 5A, EIF-5A, EIF5A1, MGC104255, MGC99547, eIF5AI, OTTHUMP00000128384, uORF A

    基因ID

    1984

    NCBI登录号

    NP_001137232

    途径

    Regulation of Muscle Cell Differentiation
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