SATB1 抗体 (Internal Region)
Quick Overview for SATB1 抗体 (Internal Region) (ABIN571004)
抗原
See all SATB1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- SATB1
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序列
- ESDEENRQKT RPRT
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交叉反应
- Cow, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-ESDEENRQKTRPRT, from the internal region of the protein sequence according to NP_002962.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Thymus shows select nuclear staining. Recommended concentration: 3-5 μg/mL.
Western Blot: Approx. 90 kDa band observed in nuclear lysates of cell lines A431 and Jurkat and in lysates of cell lines U251 and Daudi (calculated MW of 89.0 kDa according to NP_001182399.1). Recommended concentration: 0.1-0.5 μg/mL. Primary incubation 1 h
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of Jurkat cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- SATB1 (SATB Homeobox 1 (SATB1))
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别名
- SATB1
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背景
- SATB1, SATB homeobox 1, DNA-binding protein SATB1, special AT-rich sequence binding protein 1, special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA's)
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基因ID
- 6304, 20230, 316164
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NCBI登录号
- NP_002962
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途径
- Caspase Cascade in Apoptosis, Activated T Cell Proliferation
抗原
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