IL-18 抗体 (Internal Region)
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Quick Overview for IL-18 抗体 (Internal Region) (ABIN570987)
抗原
See all IL-18 (IL18) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- IL18
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序列
- NPPDNIKDTK SDI
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特异性
- This antibody is expected to recognise both reported isoforms (NP_001553.1, NP_001230140.1).
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-NPPDNIKDTKSDI, from the internal region of the protein sequence according to NP_001553.1, NP_001230140.1.
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亚型
- IgG
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anti-Interleukin 18 (IL18) (AA 37-194) antibody
IL18 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 37-193) antibodyIL18 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C14 unconjugated
anti-Interleukin 18 (IL18) (AA 37-193) antibodyIL18 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 36-192) antibodyIL18 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 94-193) antibodyIL18 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Interleukin 18 (IL18) (AA 32-192) antibody (APC)IL18 适用: 兔 WB, IHC, IP, ICC 宿主: 豚鼠 Polyclonal APC
anti-Interleukin 18 (IL18) (AA 36-192) antibodyIL18 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 37-194) antibodyIL18 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C6 unconjugated
anti-Interleukin 18 (IL18) (AA 32-192) antibodyIL18 适用: 兔 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D3 unconjugated
anti-Interleukin 18 (IL18) (AA 37-193) antibodyIL18 适用: Cow WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C7 unconjugated
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应用备注
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Western Blot: Approx 23 kDa band observed in lysates of cell line HeLa and approx. 21-22 kDa band in lysates of cell lines A431 and U2OS (calculated MW of 22.3 kDa according to NP_001553.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the golgi and cytoplasm of A431and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conce
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- IL-18 (IL18) (Interleukin 18 (IL18))
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别名
- IL18
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背景
- IL18, interleukin 18 (interferon-gamma-inducing factor), IGIF, IL-18, IL-1g, IL1F4, MGC12320, IL-1 gamma, interferon-gamma-inducing factor, interleukin 18, interleukin-1 gamma, interleukin-18
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基因ID
- 3606, 29197
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NCBI登录号
- NP_001553, NP_001230140
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途径
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
抗原
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