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SPRY1 抗体 (C-Term)

This anti-SPRY1 antibody is a 山羊 多克隆 antibody detecting SPRY1 in WB, ELISA, IHC, IF 和 FACS. Suitable for 人 和 小鼠.
产品编号 ABIN570913
发货至: 中国

Quick Overview for SPRY1 抗体 (C-Term) (ABIN570913)

抗原

See all SPRY1 抗体
SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))

适用

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人, 小鼠

宿主

  • 21
  • 6
  • 1
山羊

克隆类型

  • 24
  • 4
多克隆

标记

  • 24
  • 2
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This SPRY1 antibody is un-conjugated

应用范围

  • 23
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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

    • 9
    • 3
    • 3
    • 2
    • 2
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    • 1
    • 1
    • 1
    C-Term

    原理

    Sprouty

    序列

    CPSRGQGKPS

    特异性

    NP_005832.1 and NP_955359.1 represent varients of the same protein.

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence CPSRGQGKPS, from the C Terminus of the protein sequence according to NP_005832.1, NP_955359.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: This product has been successfully used by customers in paraffin embedded Mouse Brain, showing nuclear staining. Recommended concentration: 1-3 μg/mL.

    Western Blot: Approx 37 kDa band observed in Human Kidney lysates (calculated MW of 35.1 kDa according to NP_005832.1 ). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature. This.product has been successfully used in ERMS

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HEK293 cells. Rec

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))

    别名

    SPRY1

    背景

    SPRY1, hSPRY1, sprouty homolog 1, antagonist of FGF signaling (Drosophila), sprouty (Drosophila) homolog 1 (antagonist of FGF signaling), sprouty, Drosophila, homolog of, 1 (antagonist of FGF signaling)

    基因ID

    10252

    NCBI登录号

    NP_005832, NP_955359

    途径

    EGFR Signaling Pathway, EGFR Downregulation
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