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Desmoplakin 抗体 (Internal Region)

This 山羊 多克隆 anti-Desmoplakin antibody specifically detects Desmoplakin in IHC 和 ELISA. The antibody is reactive with 人 samples.
产品编号 ABIN570871
发货至: 中国
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Quick Overview for Desmoplakin 抗体 (Internal Region) (ABIN570871)

抗原

See all Desmoplakin (DSP) 抗体
Desmoplakin (DSP)

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This Desmoplakin antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), ELISA

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    Desmoplakin (aa 1672 -1684)

    序列

    QESVKQAHLR NEH

    特异性

    This antibody is expected to recognize reported isoform I (NP_004406.2) only.

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    C-QESVKQAHLRNEH

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Heart shows staining of the junctons between cardiomuscular fibres. Recommended concentration, 3-6 μg/mL.

    Western Blot: Preliminary experiments in lysates of cell lines HEK293, HeLa and NIH3T3 gave no specific signal but low background (at antibody concentration up to 1 μg/mL). We would appreciate any feedback from people in the field - have any results been

    Peptide ELISA: antibody detection limit dilution 1:4000.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    Desmoplakin (DSP)

    别名

    DSP

    背景

    DSP, desmoplakin, DPI, 250 kDa paraneoplastic pemphigus antigen, desmoplakin I

    基因ID

    1832, 109620, 306871

    NCBI登录号

    NP_004406

    途径

    Cell-Cell Junction Organization
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