Desmoplakin 抗体 (Internal Region)
Quick Overview for Desmoplakin 抗体 (Internal Region) (ABIN570871)
抗原
See all Desmoplakin (DSP) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- Desmoplakin (aa 1672 -1684)
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序列
- QESVKQAHLR NEH
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特异性
- This antibody is expected to recognize reported isoform I (NP_004406.2) only.
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- C-QESVKQAHLRNEH
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Heart shows staining of the junctons between cardiomuscular fibres. Recommended concentration, 3-6 μg/mL.
Western Blot: Preliminary experiments in lysates of cell lines HEK293, HeLa and NIH3T3 gave no specific signal but low background (at antibody concentration up to 1 μg/mL). We would appreciate any feedback from people in the field - have any results been
Peptide ELISA: antibody detection limit dilution 1:4000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Desmoplakin (DSP)
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别名
- DSP
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背景
- DSP, desmoplakin, DPI, 250 kDa paraneoplastic pemphigus antigen, desmoplakin I
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基因ID
- 1832, 109620, 306871
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NCBI登录号
- NP_004406
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途径
- Cell-Cell Junction Organization
抗原
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