The stated application concentrations are suggested starting points. Titration of the recombinant PLAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunohistochemistry (FFPE): 1:500-1:1000,Western Blot: 1:1000-1:2000
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Antibody in PBS with 50 % Glycerol, 1 % BSA and 0.09 % sodium azide
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Sodium azide
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This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Alkaline phosphatase, placental type is a membrane-bound glycosylated dimeric enzyme, also referred to as the heat-stable form, that is expressed primarily in the placenta, although it is closely related to the intestinal form of the enzyme as well as to the placental-like form.
PLAP is a tumor marker, especially in seminoma and ovarian cancer. PLAP is reliable only in non-smokers, as smoking interferes with measurement of PLAP, since serum concentrations of PLAP are increased up to 10-fold in smokers and its measurement is therefore of little value in this group. [Wiki]