Recombinant EIF2A 抗体 (pSer51)
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Quick Overview for Recombinant EIF2A 抗体 (pSer51) (ABIN5708157)
抗原
See all EIF2A 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer51
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原理
- Recombinant phospho-EIF2 alpha Antibody (Ser51)
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预测反应
- Mouse,Rat
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纯化方法
- Protein A purified from animal origin-free supernatant
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免疫原
- A peptide corresponding the the amino acids surrounding phosphorylated serine 51 was used as the immunogen for the recombinant phospho-EIF2 alpha antibody.
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亚型
- IgG
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anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (AA 448-576) antibody
EIF2A 适用: 人, 大鼠, 小鼠, 猴 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 3A7B11 unconjugated
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (AA 448-576) antibodyEIF2A 适用: 人 WB, IHC, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 3A7A8 unconjugated
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) antibodyEIF2A 适用: 人, 大鼠, 小鼠, 猴, 鱼 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) antibodyEIF2A 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) antibodyKD Validated EIF2A 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB4195 unconjugated Recombinant Antibody
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (pSer51), (pSer52) antibodyEIF2A 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (pSer51) antibodyKD Validated EIF2A 适用: 人 WB, FACS, IF (p) 宿主: 兔 Monoclonal 24GB3905 unconjugated Recombinant Antibody
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (AA 437-465) antibodyEIF2A 适用: 人 WB, IHC, FACS, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (AA 437-465) antibodyEIF2A 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB24073 unconjugated
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (N-Term) antibodyEIF2A 适用: 人, 大鼠, 小鼠, Pig WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- The stated application concentrations are suggested starting points. Titration of the recombinant phospho-EIF2 alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Antibody in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the recombinant phospho-EIF2 alpha antibody at -20oC.
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- EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))
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别名
- EIF2A
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背景
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Eukaryotic initiation factor 2 alpha (EIF2 alpha), encoded by the EIF2S1 gene, is a central regulator of translation initiation and a key mediator of cellular stress responses. Phospho-EIF2 alpha Antibody, clone RM298, is designed to detect EIF2 alpha phosphorylated at serine 51 (pS51), a critical regulatory site that controls global protein synthesis and activation of stress response pathways.
Phosphorylation of EIF2 alpha at Ser51 represents a pivotal switch in translational control. Under normal conditions, EIF2 alpha functions as part of the heterotrimeric eIF2 complex, delivering initiator methionyl-tRNA to the ribosome during early stages of protein synthesis. However, when Ser51 is phosphorylated, this process is inhibited, leading to a rapid reduction in global protein translation. This allows cells to conserve energy and resources while selectively translating stress-response genes that promote survival and adaptation.
The phosphorylation of EIF2 alpha is mediated by a family of stress-responsive kinases, including PERK, PKR, GCN2, and HRI. Each kinase is activated by distinct stimuli such as endoplasmic reticulum stress, viral infection, oxidative stress, or amino acid deprivation. Through these pathways, EIF2 alpha serves as a convergence point for multiple signaling networks, coordinating the integrated stress response and enabling cells to adapt to adverse conditions.
Unlike total EIF2 alpha detection, which reflects overall protein levels, phospho-specific detection at Ser51 provides direct insight into pathway activation and translational repression. Increased phosphorylation at this site is commonly observed following treatment with stress-inducing agents or phosphatase inhibitors such as Calyculin A, which enhance accumulation of the phosphorylated form. As a result, phospho-EIF2 alpha is widely used as a sensitive marker of cellular stress signaling and translational shutdown.
Subcellularly, phosphorylated EIF2 alpha is predominantly localized in the cytoplasm, where it associates with ribosomes and translation initiation complexes. Under stress conditions, it is also linked to the formation of stress granules and other ribonucleoprotein assemblies involved in mRNA storage and regulation. These structures reflect a shift from active translation to selective mRNA handling and are a hallmark of stress-induced translational control.
Dysregulation of EIF2 alpha phosphorylation has been implicated in a range of diseases, including cancer, neurodegenerative disorders, and metabolic conditions. Sustained activation of this pathway can promote tumor cell survival under hypoxic or nutrient-limited conditions, while aberrant signaling contributes to impaired protein homeostasis in neurodegeneration. Monitoring phosphorylation at Ser51 therefore provides important insight into disease mechanisms and cellular adaptation.
Phospho-EIF2 alpha Antibody, clone RM298, enables selective detection of the activated, phosphorylated form of EIF2 alpha, supporting studies of stress signaling, translational regulation, and cellular response to environmental or pharmacological stimuli. Its ability to distinguish between inactive and active states of EIF2 alpha makes it a valuable tool for investigating pathway activation and dynamic changes in protein synthesis. -
UniProt
- P05198
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途径
- Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly
抗原
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