Keratin 10 抗体
Quick Overview for Keratin 10 抗体 (ABIN5707747)
抗原
See all Keratin 10 (KRT10) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Purified
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纯度
- Protein A affinity chromatography
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免疫原
- A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the recombinant CK10 antibody.
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亚型
- IgG kappa
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应用备注
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Optimal dilution of the recombinant CK10 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1) -
限制
- 仅限研究用
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant CK10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Keratin 10 (KRT10)
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别名
- KRT10 / Cytokeratin 10
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背景
- This mAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
抗原
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