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CD44v4 抗体

适用: 人 IHC (p) 宿主: 兔 Monoclonal CDLA44v4-1R unconjugated
产品编号 ABIN5707654
发货至: 中国
  • 抗原
    CD44v4
    适用
    • 15
    • 2
    宿主
    • 12
    • 3
    • 1
    克隆类型
    • 14
    • 2
    单克隆
    标记
    • 8
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    非结合性
    应用范围
    • 13
    • 11
    • 8
    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    纯化方法
    Purified
    纯度
    Protein A affinity chromatography
    免疫原
    An amino acid sequence from the variant 4 domain of CD44 was used as the immunogen for the recombinant CD44v4 antibody.
    克隆位点
    CDLA44v4-1R
    亚型
    IgG kappa
  • 应用备注
    The stated application concentrations are suggested starting points. Titration of the recombinant CD44v4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

    1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)
    限制
    仅限研究用
  • 缓冲液
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    储存液
    Azide free
    储存条件
    4 °C,-20 °C
    储存方法
    Store the recombinant CD44v4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
  • 抗原
    CD44v4
    背景
    This antibody recognizes an epitope encoded by exon v4 on the variant portion of human CD44. The CD44 Molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. It is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.
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