Recombinant CD44v4 抗体
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北京 101111
Quick Overview for Recombinant CD44v4 抗体 (ABIN4949299)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein A affinity chromatography
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免疫原
- An amino acid sequence from the variant 4 domain of CD44 was used as the immunogen for the recombinant CD44v4 antibody.
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亚型
- IgG kappa
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anti-CD44v4 (AA 141-240) antibody
适用: 人, 小鼠 FACS, IHC (p), WB, ELISA, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD44v4 antibody适用: 人 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal CD44v4-1219 unconjugated
anti-CD44v4 antibody适用: 人 FACS, IF, IHC (p) 宿主: 兔 Monoclonal CD44v4-1700R unconjugated Recombinant Antibody
anti-CD44v4 antibody适用: 人 IHC (p) 宿主: 兔 Monoclonal CDLA44v4-1R unconjugated
anti-CD44v4 antibody适用: 人 IHC (p) 宿主: 兔 Monoclonal CDLA44v4-1R unconjugated
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应用备注
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Titering of the recombinant CD44v4 antibody may be required for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
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限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant CD44v4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- CD44v4
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背景
- This antibody recognizes an epitope encoded by exon v4 on the variant portion of human CD44. The CD44 Molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. It is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.
抗原
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