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Histone H1 抗体

This anti-Histone H1 antibody is a 兔 单克隆 antibody detecting Histone H1 in IHC (p). Suitable for 人.
产品编号 ABIN5707524
发货至: 中国

Quick Overview for Histone H1 抗体 (ABIN5707524)

抗原

See all Histone H1 抗体
Histone H1

适用

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宿主

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克隆类型

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单克隆

标记

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This Histone H1 antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

OSHT-3R
  • 纯化方法

    Purified

    纯度

    Protein A affinity chromatography

    免疫原

    Recombinant full-length human protein was used as the immunogen for the recombinant Histone H1 antibody.

    亚型

    IgG kappa
  • 应用备注

    The stated application concentrations are suggested starting points. Titration of the recombinant Histone H1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

    1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

    限制

    仅限研究用
  • 缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the recombinant Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
  • 抗原

    Histone H1

    背景

    Five major families of histones exist: H1/H5, H2A, H2B, H3, and H4. Histones H2A, H2B, H3 and H4 are known as the core histones, while histones H1/H5 are known as the linker histones. The core histones all exist as dimers, which are similar in that they all possess the histone fold domain, three alpha helices linked by two loops. It is this helical structure that allows for interaction between distinct dimers, particularly in a head-tail fashion (also called the handshake motif). The linker histone H1 binds the nucleosome at the entry and exit sites of the DNA, thus locking the DNA into place and allowing the formation of higher order structure. [Wiki]
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