Histone 3 抗体 (H3K4ac, pThr3)
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- 抗原 See all Histone 3 (H3) 抗体
- Histone 3 (H3)
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抗原表位
- H3K4ac, pThr3
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适用
- 人, 小鼠, C. elegans
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Histone 3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Multiplex Assay (MA), Fluorescence Microscopy (FM)
- Supplier Product No.
- 600-401-i56
- Supplier
- Rockland
- 原理
- Histone H3 phospho T3/K4ac Antibody
- 交叉反应 （详细）
- This antibody reacts with human Histone H3.
- 产品特性
- Synonyms: rabbit anti-Histone H3 Ac Lys4 pT3 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87782, MGC87783, H3pT3/K4ac
- 纯化方法
- Anti-Histone H3 [ac Lys4, p Thr3] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Histone H3 [ac Lys4, p Thr3] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic acetylated/phosphorylated peptide surrounding Lysine 4 and Threonine 3 of human Histone H3.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product H3 Primary Antibody
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anti-Histone 3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27ac) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (3meLys4) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K36me3) antibodyH3 适用: 人, 小鼠 WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 小鼠 Monoclonal MABI 0333 unconjugated
anti-Histone 3 (H3) (H3K9ac) antibodyH3 适用: 人, 小鼠 WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (AA 71-136) antibodyH3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IHC (p), IF (cc), FACS, IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27me) antibodyH3 适用: 人 WB, IF, IHC, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K36me3) antibodyH3 适用: 人 WB, IF, IHC, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K36me2) antibodyH3 适用: 人 WB, IF, IHC, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:50
Application Note: Anti-Histone H3 [ac Lys4, p Thr3] antibody is tested for Western Blot, Immunocytochemistry/Immunofluorescence, and Dot Blot. This antibody is useful in Chromatin Immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
ChIP Dilution: 2-5 μg/million cells
Western Blot Dilution: 1:500
IF Microscopy Dilution: 1:50
Other: Dot Blot 1:1000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.57 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Histone 3 (H3)
- 别名
- Histone H3 (H3 产品)
- 背景
- Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Phosphorylation of threonine 3 (H3 pT3) is a known mitotic marker and modified by the Haspin/Thr3 enzyme, while acetylation of lysine 4 (H3K4ac) on histone 3 is associated with transcriptional activation by Esa1. Methylation that occurs on H3K4 concurrently with acetylation seems to act as an adjuster to the activation effects of acetylation. Shugoshin protein cannot bind to the centromere of active cells when H3K4 is acetylated, which reduces dimethylation, and thus slows meiosis and mitosis. Usually, H3K4Ac is a transitional modification, and will become further modified with methylation as transcription progresses, indicating complex transcriptional regulation. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
- 基因ID
- 126961
- NCBI登录号
- NP_001005464
- UniProt
- Q71DI3
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