EZH1 抗体
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- 抗原 See all EZH1 抗体
- EZH1 (Enhancer of Zeste Homolog 1 (EZH1))
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This EZH1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 600-401-gt9
- Supplier
- Rockland
- 原理
- EZH1 Antibody
- 交叉反应 (详细)
- Anti-EZH1 is directed against human EZH1 at an internal position.
- 产品特性
- Synonyms: rabbit anti-EZH1 antibody, KIAA0388, Histone-lysine N-methyltransferase EZH1, ENX-2, Enhancer of zeste homolog 1, EZH-1, EZH 1
- 纯化方法
- This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Affinity purified Anti-EZH1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human EZH1 protein.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product EZH1 Primary Antibody
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- 应用备注
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Application Note: Affinity purified Anti-EZH1 antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~86 kDa in size corresponding to Ezh1 by western blotting in the appropriate cell lysate or extract. Testing using positive control NIH/3T3 nuclear extract p/n W10-001-A74.
Western Blot Dilution: 1.0 μg/mL
ELISA Dilution: 5.0 μg/mL
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- EZH1 (Enhancer of Zeste Homolog 1 (EZH1))
- 别名
- EZH1 (EZH1 产品)
- 背景
- Background: Anti-Ezh1 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Anti-EZH1 Antibody detects human EZH1. Polycomb group (PcG) proteins are essential epigenetic regulators in normal tissue homeostasis and are involved in transcriptional repression. The PcG members Ezh2 and Ezh1 are important determinants of embryonic stem cell identity, and the transcript levels of these histone methyltransferases are inversely correlated during development. Recent studies have shown that EZH1 also has histone H3K27 methyltransferase activity and binds to an overlapping subset of genes. EZH1 and EZH2 have different expression patterns. EZH2 is found in actively proliferating cells, whereas EZH1 expression is higher in nonproliferative adult tissues. EZH1 partially compensates for the loss of EZH2, as shown in cells lacking only Ezh2. In mice, EZH1 is a regulator of homeotic gene expression implicated in the assembly of repressive protein complexes in chromatin. Anti-EZH1 Antibody is ideal for investigators studying pathogenesis of hematological malignancies.
- 基因ID
- 2145
- NCBI登录号
- NP_001982
- UniProt
- Q92800
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