Leptin 抗体
Quick Overview for Leptin 抗体 (ABIN5706824)
抗原
See all Leptin (LEP) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 210-401-320-0100
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Supplier
- Rockland
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原理
- Leptin Antibody
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交叉反应 （详细）
- The antibody will recognize leptin from mouse and human.
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产品特性
- Synonyms: rabbit anti-leptin antibody, LEP antibody, Leptin Murine Obesity Homolog antibody, Leptin Precursor Obesity Factor antibody, OB antibody, Obese Protein antibody
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纯化方法
- Anti-Leptin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Leptin antibody was prepared by repeated immunizations with recombinant mouse leptin 16,000 MW produced in E. coli.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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anti-Leptin (LEP) (AA 22-167) antibody
LEP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 22-167) antibodyLEP 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 17-100) antibodyLEP 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 8-37) antibodyLEP 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 22-167) antibodyLEP 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 29-160) antibodyLEP 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 22-167) antibodyLEP 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 7-37), (N-Term) antibodyLEP 适用: 人 WB, IF, FACS, EIA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 101-154) antibodyLEP 适用: 小鼠, 大鼠 ELISA, IF (p), IF (cc), FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 22-167) antibodyLEP 适用: 人 WB 宿主: 小鼠 Monoclonal C5 unconjugated
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: This IgG fraction of anti-Mouse Leptin was tested for use in ELISA and immunoblotting. Reactivity in other immunoassays is unknown. The antibody will recognize recombinant and native 16 kDa leptin from mouse and human.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Leptin (LEP)
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别名
- Lep
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背景
- Background: Anti-Leptin antibody detects leptin protein. Leptin may function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Obesity associated leptin is the product of the OB gene and has been identified with Type II diabetes. Anti-leptin Antibody is ideal for investigators involved in Cell Signaling, Obesity research, aging research and Signal Transduction research.
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基因ID
- 16846
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UniProt
- P41160
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途径
- JAK/STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
抗原
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