Histone 3 抗体 (H3R2me2a)
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- 抗原 See all Histone 3 (H3) 抗体
- Histone 3 (H3)
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抗原表位
- H3R2me2a
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适用
- 人, C. elegans
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Histone 3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Dot Blot (DB), Fluorescence Microscopy (FM)
- Supplier Product No.
- 600-401-i53
- Supplier
- Rockland
- 原理
- Histone H3 R2me2a Antibody
- 交叉反应 （详细）
- This antibody reacts with human Histone H3.
- 产品特性
- Synonyms: rabbit anti-Histone H3 Asym-dimethyl Arg2 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3R2me2a
- 纯化方法
- Anti-Histone H3 [Asym-dimethyl Arg2] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Histone H3 [Asym-dimethyl Arg2] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Arginine 2 of human Histone H3.2.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product H3 Primary Antibody
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anti-Histone 3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27ac) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (3meLys4) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K36me3) antibodyH3 适用: 人, 小鼠 WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 小鼠 Monoclonal MABI 0333 unconjugated
anti-Histone 3 (H3) (H3K9ac) antibodyH3 适用: 人, 小鼠 WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (AA 71-136) antibodyH3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IHC (p), IF (cc), FACS, IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27me) antibodyH3 适用: 人 WB, IF, IHC, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K36me3) antibodyH3 适用: 人 WB, IF, IHC, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K36me2) antibodyH3 适用: 人 WB, IF, IHC, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:50-1:100
Application Note: Anti-Histone H3 [Asym-dimethyl Arg2] antibody is tested by Western Blot, Dot Blot, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
Western Blot Dilution: 1:500
IF Microscopy Dilution: 1:50-1:100
Other: Dot Blot 1:1000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.71 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Histone 3 (H3)
- 别名
- Histone H3 (H3 产品)
- 背景
- Background: When Set1 attempts to methylate H3K4, epigenetic silencing of downstream genes is mediated by dimethylation of histone H3 at Arg2. This modification is typically found in heterochromatin and inactive genes, and is not found when trimethylation of Arg2 is observed. Conversion from mono-, di- and tri-methyl forms of H3R2 is mediated through the activity of the arginine methyltransferase PRMT6. H3K4 cannot be methylated when the asymmetric H3R2me2 mark is present. In active promoters, this modification is actively eliminated. H3R2me2a prevents WDR5 recognition, MLL methyltransferase recruitment, and H3K4 methylation. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
- 基因ID
- 126961
- NCBI登录号
- NP_001005464
- UniProt
- Q71DI3
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