Histone 3 抗体 (H3K79me3)
Quick Overview for Histone 3 抗体 (H3K79me3) (ABIN5706779)
抗原
See all Histone 3 (H3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- H3K79me3
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Supplier Product No.
- 600-401-i95
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Supplier
- Rockland
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原理
- Histone H3 K79me3 Antibody
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交叉反应 （详细）
- This antibody reacts with human Histone H3.
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产品特性
- Synonyms: rabbit anti-Histone H3 trimethyl Lys79 antibody, H3.3B, H3K27Me3, H3K79Me3, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782
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纯化方法
- Anti-Histone H3 [Trimethyl Lys79] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Histone H3 [Trimethyl Lys79] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic trimethylated peptide surrounding Lysine 79 of human Histone H3.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Histone 3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27ac) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (3meLys4) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K36me3) antibodyH3 适用: 人, 小鼠 WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 小鼠 Monoclonal MABI 0333 unconjugated
anti-Histone 3 (H3) (H3K9ac) antibodyH3 适用: 人, 小鼠 WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (AA 71-136) antibodyH3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (C-Term) antibodyH3 适用: 人 WB, IHC, ChIP, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 适用: 人 WB, IF, IHC, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K9me2) antibodyH3 适用: 人 WB, IF, IHC, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:100-1:1000
Application Note: Anti-Histone H3 [Trimethyl Lys79] antibody is tested in Western Blot, Dot Blot, Chromatin Immunoprecipitation, and Immunofluorescence. This antibody is useful for Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
ChIP Dilution: 2-5 μg/million cells
Western Blot Dilution: 1 μg/mL
IF Microscopy Dilution: 1:100-1:1000
Other: user optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.6 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Histone 3 (H3)
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别名
- Histone H3
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背景
- Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by histone modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways. Anti-Histone H3 are ideal for researchers interested in Chromatin Research, Epigenetics, Chromatin Modifiers, Histones and Modified Histones, and Phospho Specific research.
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基因ID
- 126961
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NCBI登录号
- NP_001005464
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UniProt
- Q71DI3
抗原
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