Histone 3 抗体 (H3K36me)
Quick Overview for Histone 3 抗体 (H3K36me) (ABIN5706763)
抗原
See all Histone 3 (H3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- H3K36me
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Supplier Product No.
- 600-401-i86
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Supplier
- Rockland
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原理
- Histone H3 K36me1 Antibody
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交叉反应 （详细）
- This antibody reacts with human Histone H3.
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产品特性
- Synonyms: rabbit anti-Histone H3 monomethyl Lys36 antibody, H3.3B, H3.3AH3F3H3F3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3K36me1
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纯化方法
- Anti-Histone H3 [Monomethyl Lys36] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Histone H3 [Monomethyl Lys36] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 36 of human Histone H3.2.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Histone 3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K27ac) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (3meLys4) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K36me3) antibodyH3 适用: 人, 小鼠 WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 小鼠 Monoclonal MABI 0333 unconjugated
anti-Histone 3 (H3) (H3K9ac) antibodyH3 适用: 人, 小鼠 WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (AA 71-136) antibodyH3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (C-Term) antibodyH3 适用: 人 WB, IHC, ChIP, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K4me3) antibodyH3 适用: 人 WB, IF, IHC, ChIP, IP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone 3 (H3) (H3K9me2) antibodyH3 适用: 人 WB, IF, IHC, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:500
Application Note: Anti-Histone H3 [Monomethyl Lys36] antibody is tested in Western Blot, Dot Blot, and Immunofluorescence. This antibody is useful for Chromatin Immunoprecipitation and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
ChIP Dilution: 2-5 μg/million cells
Western Blot Dilution: 1:1000
IF Microscopy Dilution: 1:500
Other: Dot Blot 0.5 μg/mL
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.45 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Histone 3 (H3)
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别名
- Histone H3
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背景
- Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Conversion from trimethyl to di- and singly methylated forms also occurs via the transcriptional repressor JHDM3A. H3K36Me1 has been associated with the timing of replication factor Cdc45 association with replicating origins. H3K36Me1 could have important influence over the conserved multiprotein complex and minichromosome maintenance proteins. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
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基因ID
- 126961
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NCBI登录号
- NP_001005464
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UniProt
- Q71DI3
抗原
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