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- 抗原 See all RNF43 抗体
- RNF43 (Ring Finger Protein 43 (RNF43))
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抗原表位
- AA 24-197
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RNF43 antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB)
- 原理
- Anti-RNF43 Antibody Picoband®
- 交叉反应 （详细）
- No cross-reactivity with other proteins.
- 产品特性
- Anti-RNF43 Antibody Picoband® (ABIN5693038). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 免疫原
- E. coli-derived human RNF43 recombinant protein (Position: G24-Y197).
- 亚型
- IgG
- Top Product
- Discover our top product RNF43 Primary Antibody
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anti-Ring Finger Protein 43 (RNF43) (AA 41-140) antibody
RNF43 适用: 人, 小鼠 ELISA, WB, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 43 (RNF43) (AA 543-572), (C-Term) antibodyRNF43 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB32930 unconjugated
anti-Ring Finger Protein 43 (RNF43) (AA 543-572) antibodyRNF43 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 43 (RNF43) (AA 566-697) antibodyRNF43 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 43 (RNF43) (AA 504-553) antibodyRNF43 适用: 人, 小鼠, Cow, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger Protein 43 (RNF43) (Middle Region) antibodyRNF43 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western blot,0.1-0.5 μg/mL
ELISA,0.1-0.5 μg/mL
1. Sugiura, T., Yamaguchi, A., Miyamoto, K. A cancer-associated RING finger protein, RNF43, is a ubiquitin ligase that interacts with a nuclear protein, HAP95. Exp. Cell Res. 314: 1519-1528, 2008. 2. Yagyu, R., Furukawa, Y., Lin, Y.-M., Shimokawa, T., Yamamura, T., Nakamura, Y. A novel oncoprotein RNF43 functions in an autocrine manner in colorectal cancer. Int. J. Oncol. 25: 1343-1348, 2004. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- 抗原
- RNF43 (Ring Finger Protein 43 (RNF43))
- 别名
- RNF43 (RNF43 产品)
- 背景
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Synonyms: E3 ubiquitin-protein ligase RNF43, RING finger protein 43, RING-type E3 ubiquitin transferase RNF43, RNF43
Tissue Specificity: Expressed in fetal kidney, fetal lung, in colon cancer tissues, hepatocellular carcinomas and lung adenocarcinomas. Overexpressed in colorectal cancer cell lines.
Background: RNF43 is a HAP95 (AKAP8L) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer. The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms.
- 分子量
- 86 kDa
- 基因ID
- 54894
- 途径
- WNT signaling
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