PARN 抗体 (AA 1-301)
Quick Overview for PARN 抗体 (AA 1-301) (ABIN5693032)
抗原
See all PARN 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-301
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原理
- Anti-PARN Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-PARN Antibody Picoband® (ABIN5693032). Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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免疫原
- E. coli-derived human PARN recombinant protein (Position: M1-Y301).
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亚型
- IgG
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anti-Poly A Specific Ribonuclease (PARN) (AA 1-639) antibody
PARN 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Poly A Specific Ribonuclease (PARN) (AA 1-639) antibodyPARN 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Poly A Specific Ribonuclease (PARN) antibodyPARN 适用: 人, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Poly A Specific Ribonuclease (PARN) (AA 501-599) antibodyPARN 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Poly A Specific Ribonuclease (PARN) antibodyPARN 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Poly A Specific Ribonuclease (PARN) (AA 1-280) antibodyPARN 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Poly A Specific Ribonuclease (PARN) antibodyPARN 适用: 人 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Poly A Specific Ribonuclease (PARN) antibodyPARN 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Poly A Specific Ribonuclease (PARN) (N-Term) antibodyPARN 适用: 人, 小鼠, 大鼠, Cow, 犬, 猴, 豚鼠, 马, 兔, Bat, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-Poly A Specific Ribonuclease (PARN) (C-Term) antibodyPARN 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. "Entrez Gene: PARN poly(A)-specific ribonuclease (deadenylation nuclease)". 2. Buiting K, Korner C, Ulrich B, Wahle E, Horsthemke B (May 2000). "The human gene for the poly(A)-specific ribonuclease (PARN) maps to 16p13 and has a truncated copy in the Prader-Willi/Angelman syndrome region on 15q11→q13". Cytogenet Cell Genet. 87 (1-2): 125-31. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- PARN (Poly A Specific Ribonuclease (PARN))
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别名
- PARN
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背景
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Synonyms: Poly (A)-specific ribonuclease PARN, Deadenylating nuclease, Deadenylation nuclease, Polyadenylate-specific ribonuclease, PARN, DAN
Tissue Specificity: Ubiquitous.
Background: Poly (A)-specific ribonuclease (PARN), also known as polyadenylate-specific ribonuclease or deadenylating nuclease (DAN), is an enzyme that in humans is encoded by the PARN gene. The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly (A) as the substrate, hence, efficiently degrades poly (A) tails of mRNAs. Exonucleolytic degradation of the poly (A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons.
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分子量
- 78 kDa
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基因ID
- 5073
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UniProt
- O95453
抗原
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