Desmin 抗体
Quick Overview for Desmin 抗体 (ABIN5691092)
抗原
See all Desmin (DES) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The antibody D33 reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma in human, hamster, rat and chicken. The antibody does not cross react with vimentin or any of the other intermediate filament proteins.
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免疫原
- D33 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a BALB/c mouse immunized with purified desmin from a leiomyosarcoma.
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亚型
- IgG1
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anti-Desmin (DES) (C-Term) antibody
Verified DES 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Desmin (DES) (AA 1-304) antibodyDES 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Desmin (DES) (AA 261-360) antibodyDES 适用: 人, 大鼠, 小鼠, 小鸡 WB, ELISA, IHC (p), FACS, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Desmin (DES) antibodyDES 适用: 人 WB, IF, IHC (p), FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Desmin (DES) (AA 121-470) antibodyDES 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Desmin (DES) antibodyDES 适用: 人, 大鼠, 小鼠, 小鸡, Hamster, Pig, 犬, 兔, 斑马鱼 WB, IHC, IHC (fro) 宿主: 小鼠 Monoclonal RD301 unconjugated
anti-Desmin (DES) antibodyDES 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 小鼠 Monoclonal 3E1G4 unconjugated
anti-Desmin (DES) (C-Term) antibodyDES 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Desmin (DES) (AA 361-470) antibodyDES 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D12 unconjugated
anti-Desmin (DES) antibodyDES 适用: 人, 大鼠, 小鼠, Hamster, Pig, 犬, 兔 WB, IHC, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal unconjugated
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应用备注
- The D33 antibody is suitable for the detection of desmin by Western blotting and detects a band of 53 kDa in muscle tissue extracts. The antibody is also suitable for the detection of desmin by immunocytochemistry on permeabilized cells, and Immunohistochemistry on frozen and formalin fixed, paraffin embedded tissues. The antibody works on paraffin-embedded material without pretreament with proteolytic enzymes, but the section should be pre-treated using heat mediated antigen retrieval with Tris-buffer containing 1 mM EDTA, pH 9,0 for 20 mins. Optimal antibody dilutions for the different applications should be determined by titration.
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限制
- 仅限研究用
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缓冲液
- Each vial contains 100 μL 1 mg/mL purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C, or in small aliquots at -20°C.
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- Desmin (DES)
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别名
- Desmin
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背景
- Desmin is a 53 kDa intermediate filament protein that exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.
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UniProt
- P17661
抗原
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