Tollip antibody can be used for detection of human and mouse Tollip by immunoprecipitation and western blot (5μ,g/mL). Method: RIPA buffer extracts from 1.5x106 EL-4 cells were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with Kimmy-1 at a dilution of 5 μ,g/mL. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.
限制
仅限研究用
状态
Liquid
缓冲液
PBS containing 0.02 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Tollip antibody can be stored at 4°C, stable for one year.