Perforin 1 抗体 (AA 98-534)
Quick Overview for Perforin 1 抗体 (AA 98-534) (ABIN5690663)
抗原
See all Perforin 1 (PRF1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 98-534
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纯化方法
- Perforin (monoclonal CB5.4) antibody was purified to ≥,95% in SDS-PAGE.
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免疫原
- Perforin (monoclonal CB5.4) antibody was raised against a recombinant mouse perforin, amino acids 98-534.
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亚型
- IgG2a
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anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 280-555) antibody
PRF1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) antibodyPRF1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 461-555) antibodyPRF1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3B4 unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) antibodyPRF1 适用: 人, Cow IP, FACS, ICC, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal dG9 unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 40-355) antibodyPRF1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 413-552) antibodyPRF1 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal PRF1-2470 unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) antibody (FITC)PRF1 适用: 人, Cow ICFC 宿主: 小鼠 Monoclonal dG9 FITC
anti-Perforin 1 (Pore Forming Protein) (PRF1) antibody (APC)PRF1 适用: 人, Cow ICFC 宿主: 小鼠 Monoclonal dG9 APC
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 32-316) antibodyPRF1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 21-554) antibodyPRF1 适用: 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Perforin antibody recognizes only membrane bound active (non-cleaved) human FasL and not the inactive soluble FasL. This product can be used for detection of active human FasL by flow cytometry and western blot. Method: A mouse T cell clone was plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washings in PBS, 0.1 % BSA, slides were incubated with 5 μ,g/mL of CB5.4 antibody, washed in PBS, incubated in anti-rat IgG-HRP, washed in PBS, and developed in appropriate substrate solution (DAB or AEC). Method: Cell extracts from the T cell clone B6.1 (2x106) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 μ,g/mL. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS containing 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Perforin antibody can be stored at 4°C, stable for one year.
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- Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))
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别名
- Perforin
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基因ID
- 18646
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UniProt
- P10820
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途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
-
