Betacellulin 抗体 (Biotin)
Quick Overview for Betacellulin 抗体 (Biotin) (ABIN5690379)
抗原
See all Betacellulin (BTC) 抗体适用
宿主
克隆类型
标记
应用范围
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免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBetacellulin. Human Betacellulin specific antibody was purified by affinity chromatography and then biotinylated.
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应用备注
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ELISA:
Direct:
To detect hBetacellulin by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin.
Sandwich
To detect hBetacellulin by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human Betacellulin as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin.
Western Blot:
To detect hBetacellulin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBetacellulin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- Betacellulin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- Betacellulin (BTC)
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别名
- Betacellulin
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背景
- Betacellulin (BTC) is a heparin-binding member of the EGF family of growth factors that includes epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), amphiregulin (AR), heparin-binding EGF-like growth factor (HB-EGF), and the various heregulins (HRG).
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基因ID
- 685
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UniProt
- P35070
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
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