MIB1 antibody can be used for detection of MIB1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunocytochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
MIB1 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
MIB1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
抗原
MIB1
(Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))
别名
MIB1
背景
The E3 ubiquitin-protein ligase MIB1, also known as Mindbomb homolog 1, contains multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase (1). MIB1 interacts with and regulates the cellular expression of the death-associated protein kinase 1 (DAPK1) protein by promoting its ubiquitination and degradation (1). It also positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis (2).