EGF 抗体 (pSer1070)
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Quick Overview for EGF 抗体 (pSer1070) (ABIN5689757)
抗原
See all EGF 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer1070
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特异性
- This antibody detects endogenous level of EGFR only when phosphorylated at serine1070.
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纯化方法
- Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
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免疫原
- EGFR (Phospho-Ser1070) antibody was raised against a peptide sequence around phosphorylation site of serine 1070 (R-Y-S (p) -S-D) derived from Human EGFR.
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应用备注
- Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store antibody at -20°C for up to one year.
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- EGF (Epidermal Growth Factor (EGF))
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别名
- EGF
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背景
- Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
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分子量
- 175 kDa
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基因ID
- 1956
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NCBI登录号
- NP_005219
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UniProt
- P00533
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途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
抗原
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