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EIF2A 抗体 (AA 49-53)
This anti-EIF2A antibody is a 兔 多克隆 antibody detecting EIF2A in WB, IHC 和 IF. Suitable for 人, 小鼠 和 大鼠.
Quick Overview for EIF2A 抗体 (AA 49-53) (ABIN5689397)
抗原
See all EIF2A 抗体
EIF2A
(Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))
适用
All reactivities for EIF2A 抗体
人, 小鼠, 大鼠
宿主
All hosts for EIF2A 抗体
兔
克隆类型
All clonalities for EIF2A 抗体
多克隆
标记
All conjugates for EIF2A 抗体
This EIF2A antibody is un-conjugated
应用范围
All applications for EIF2A 抗体
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
EIF2A抗体详情
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抗原表位
All epitopes for EIF2A 抗体
AA 49-53
特异性
This antibody detects endogenous level of total eIF2α, protein.
纯化方法
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
免疫原
eIF2a (Ab-51) antibody was raised against a peptide sequence around aa.49~53 (E-L-S-R-R) derived from Human eIF2α,.
Alternatives
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使用细节
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应用备注
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
限制
仅限研究用
贮存及处理
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状态
Liquid
浓度
1 mg/mL
缓冲液
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Store antibody at -20°C for up to one year.
EIF2A目标详情
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抗原
EIF2A
(Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))
别名
eIF2a
背景
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
分子量
38 kDa
基因ID
1965
NCBI登录号
NP_004085
UniProt
P05198
途径
Ribonucleoprotein Complex Subunit Organization , ER-Nucleus Signaling , Hepatitis C , Methionine Biosynthetic Process , Ribosome Assembly
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原发性抗体
anti-EIF2A 抗体 (AA 49-53) (ABIN5689397)
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