EIF2A 抗体 (AA 49-53)
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Quick Overview for EIF2A 抗体 (AA 49-53) (ABIN5689397)
抗原
See all EIF2A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 49-53
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特异性
- This antibody detects endogenous level of total eIF2α, protein.
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纯化方法
- Antibodies were purified by affinity-chromatography using epitope-specific peptide.
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免疫原
- eIF2a (Ab-51) antibody was raised against a peptide sequence around aa.49~53 (E-L-S-R-R) derived from Human eIF2α,.
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应用备注
- Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store antibody at -20°C for up to one year.
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- EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))
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别名
- eIF2a
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背景
- Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
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分子量
- 38 kDa
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基因ID
- 1965
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NCBI登录号
- NP_004085
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UniProt
- P05198
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途径
- Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly
抗原
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