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CCL3 抗体

This anti-CCL3 antibody is a 兔 多克隆 antibody detecting CCL3 in WB, ELISA 和 Neut. Suitable for 大鼠.
产品编号 ABIN5689196
发货至: 中国

Quick Overview for CCL3 抗体 (ABIN5689196)

抗原

See all CCL3 抗体
CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

适用

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大鼠

宿主

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克隆类型

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多克隆

标记

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This CCL3 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 纯化方法

    Anti-Rat MIP-1alpha specific antibody was purified by affinity chromatography employing immobilized Rat MIP-1alpha matrix.

    免疫原

    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat MIP-1alpha (Macrophage Inflammatory Protein -1 alpha).
  • 应用备注

    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of Rat MIP-1α, (100 ng/mL), a concentration of 8.0 - 10.0 μ,g/mL of this antibody is required.

    ELISA:

    To detect Rat MIP-1α, by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat MIP-1α,.

    Sandwich:

    To detect Rat MIP-1α, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat MIP-1α, (XP-5233Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MIP-1α,.

    Western Blot:

    To detect Rat MIP-1α, by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MIP-1α, is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    -20 °C

    储存方法

    MIP-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

    别名

    MIP-1a

    背景

    Macrophage Inflammatory Protein 1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77 % and 70 % amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.

    基因ID

    25542

    UniProt

    P50229

    途径

    Cellular Response to Molecule of Bacterial Origin, Autophagy
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