CCL3 抗体 (Biotin)
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北京 101111
Quick Overview for CCL3 抗体 (Biotin) (ABIN5689195)
抗原
See all CCL3 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Anti-rat MIP-1-alpha specific antibody was purified by affinity chromatography and then biotinylated.
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免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat MIP-1-alpha (rat MIP-1-alpha).
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应用备注
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ELISA:
To detect rat MIP-1-α, by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant rat MIP-1-α,.
Sandwich:
To detect Rat MIP-1α, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat MIP-1α, (XP-5233) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MIP-1α,.
Western Blot:
To detect rat MIP-1-α, by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat MIP-1-α, is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- MIP-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
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别名
- MIP-1a
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背景
- Macrophage Inflammatory Protein 1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77 % and 70 % amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.
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基因ID
- 25542
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UniProt
- P50229
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途径
- Cellular Response to Molecule of Bacterial Origin, Autophagy
抗原
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