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CCL2 抗体

This anti-CCL2 antibody is a 兔 多克隆 antibody detecting CCL2 in WB, ELISA 和 Neut. Suitable for 大鼠.
产品编号 ABIN5689194
发货至: 中国

Quick Overview for CCL2 抗体 (ABIN5689194)

抗原

See all CCL2 抗体
CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

适用

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大鼠

宿主

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克隆类型

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多克隆

标记

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This CCL2 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 纯化方法

    Anti-Rat MCP-1/MCAF specific antibody was purified by affinity chromatography employing immobilized Rat MCP-1/MCAF matrix.

    免疫原

    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat MCP-1/MCAF (Macrophage/Monocyte chemotactic protein-1).
  • 应用备注

    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of Rat MCP-1/MCAF (100 ng/mL), a concentration of 2.0 - 4.0 μ,g/mL of this antibody is required.

    ELISA:

    To detect Rat MCP-1/MCAF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat MCP-1/MCAF.

    Sandwich:

    To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat MCP-1(MCAF) (XP-5219Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MCP-1(MCAF).

    Western Blot:

    To detect Rat MCP-1/MCAF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1/MCAF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    -20 °C

    储存方法

    MCP-1/MCAF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

    别名

    MCP-1/MCAF

    基因ID

    24770

    UniProt

    P14844

    途径

    Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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