CCL2 抗体
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北京 101111
Quick Overview for CCL2 抗体 (ABIN5689194)
抗原
See all CCL2 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Anti-Rat MCP-1/MCAF specific antibody was purified by affinity chromatography employing immobilized Rat MCP-1/MCAF matrix.
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免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat MCP-1/MCAF (Macrophage/Monocyte chemotactic protein-1).
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat MCP-1/MCAF (100 ng/mL), a concentration of 2.0 - 4.0 μ,g/mL of this antibody is required.
ELISA:
To detect Rat MCP-1/MCAF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat MCP-1/MCAF.
Sandwich:
To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat MCP-1(MCAF) (XP-5219Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MCP-1(MCAF).
Western Blot:
To detect Rat MCP-1/MCAF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1/MCAF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- MCP-1/MCAF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
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别名
- MCP-1/MCAF
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基因ID
- 24770
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UniProt
- P14844
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途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
抗原
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