VEGF 抗体
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北京 101111
Quick Overview for VEGF 抗体 (ABIN5689180)
抗原
See all VEGF 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Anti-mVEGF specific antibody was purified by affinity chromatography employing immobilized mVEGF matrix.
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免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mVEGF (murine VEGF).
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mVEGF (50.00 ng/mL), a concentration of 1.0 2.0 μ,g/mL of this antibody is required.
ELISA:
To detect mVEGF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mVEGF.
Sandwich:
To detect mVEGF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine VEGF (XP-5293Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mVEGF.
Western Blot:
To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- VEGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- VEGF (Vascular Endothelial Growth Factor (VEGF))
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别名
- VEGF
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基因ID
- 22339
抗原
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