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KIT Ligand 抗体

This anti-KIT Ligand antibody is a 兔 多克隆 antibody detecting KIT Ligand in WB, ELISA 和 Neut. Suitable for 小鼠.
产品编号 ABIN5689173
发货至: 中国

Quick Overview for KIT Ligand 抗体 (ABIN5689173)

抗原

See all KIT Ligand (KITLG) 抗体
KIT Ligand (KITLG)

适用

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小鼠

宿主

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克隆类型

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多克隆

标记

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This KIT Ligand antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 纯化方法

    Anti-mSCF specific antibody was purified by affinity chromatography employing immobilized mSCF matrix.

    免疫原

    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mSCF (murine SCF).
  • 应用备注

    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of mSCF (25.0 ng/mL), a concentration of 0.6 - 0.65 μ,g/mL of this antibody is required.

    ELISA:

    To detect mSCF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mSCF.

    Sandwich:

    To detect mSCF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine SCF (XP-5266Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSCF.

    Western Blot:

    To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    -20 °C

    储存方法

    SCF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    KIT Ligand (KITLG)

    别名

    SCF

    基因ID

    17311

    UniProt

    P20826

    途径

    RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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