KIT Ligand 抗体
Quick Overview for KIT Ligand 抗体 (ABIN5689173)
抗原
See all KIT Ligand (KITLG) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
纯化方法
- Anti-mSCF specific antibody was purified by affinity chromatography employing immobilized mSCF matrix.
-
免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mSCF (murine SCF).
-
-
-
-
应用备注
-
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mSCF (25.0 ng/mL), a concentration of 0.6 - 0.65 μ,g/mL of this antibody is required.
ELISA:
To detect mSCF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mSCF.
Sandwich:
To detect mSCF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine SCF (XP-5266Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mSCF.
Western Blot:
To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
储存条件
- -20 °C
-
储存方法
- SCF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
-
-
- KIT Ligand (KITLG)
-
别名
- SCF
-
基因ID
- 17311
-
UniProt
- P20826
-
途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
-