JE 抗体 (Biotin)
The 兔 多克隆 anti-JE antibody is suitable to detect JE in samples from 小鼠. It has been validated for ELISA 和 WB.
产品编号 ABIN5689156
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for JE 抗体 (Biotin) (ABIN5689156)
抗原
JE
适用
小鼠
宿主
兔
克隆类型
多克隆
标记
Biotin
应用范围
ELISA, Western Blotting (WB)
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纯化方法
- Anti-mJE specific antibody was purified by affinity chromatography and then biotinylated.
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免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mJE (murine JE).
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应用备注
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ELISA:
To detect mJE by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mJE.
Sandwich:
To detect mJE(MCP-1) by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine JE(MCP-1) (XP-5206) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mJE(MCP-1).
Western Blot:
To detect mJE by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mJE is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- JE(MCP-1) antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- JE
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基因ID
- 20296
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UniProt
- P10148
抗原
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