JE 抗体
This 兔 多克隆 antibody specifically detects JE in ELISA, WB 和 Neut. It exhibits reactivity toward 小鼠.
产品编号 ABIN5689155
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for JE 抗体 (ABIN5689155)
抗原
JE
适用
小鼠
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
ELISA, Western Blotting (WB), Neutralization (Neut)
-
-
纯化方法
- Anti-mJE (MCP-1) specific antibody was purified by affinity chromatography employing immobilized mJE (MCP-1) matrix.
-
免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mJE (MCP-1) (murine JE (Macrophage/Monocyte chemotactic protein-1)).
-
-
-
-
应用备注
-
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mJE (MCP-1) (100 ng/mL), a concentration of 4.0 - 6.0 μ,g/mL of this antibody is required.
ELISA:
To detect mJE (MCP-1) by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mJE (MCP-1).
Sandwich:
To detect mJE(MCP-1) by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine JE(MCP-1)(XP-5206Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mJE(MCP-1).
Western Blot:
To detect mJE (MCP-1) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mJE (MCP-1) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
储存条件
- -20 °C
-
储存方法
- JE(MCP-1) antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
-
-
- JE
-
基因ID
- 20296
-
UniProt
- P10148
抗原
-
You are here:
