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IL-7 抗体

This anti-IL-7 antibody is a 兔 多克隆 antibody detecting IL-7 in WB, ELISA 和 Neut. Suitable for 小鼠.
产品编号 ABIN5689150
发货至: 中国

Quick Overview for IL-7 抗体 (ABIN5689150)

抗原

See all IL-7 (IL7) 抗体
IL-7 (IL7) (Interleukin 7 (IL7))

适用

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小鼠

宿主

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克隆类型

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多克隆

标记

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This IL-7 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 纯化方法

    Anti-mIL-7 specific antibody was purified by affinity chromatography employing immobilized mIL-7 matrix.

    免疫原

    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-7 (murine IL-7).
  • 应用备注

    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of mIL-7 (0.60 ng/mL), a concentration of 0.012 - 0.02 μ,g/mL of this antibody is required.

    ELISA:

    To detect mIL-7 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-7.

    Sandwich:

    To detect mIL-7 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IL-7 (XP-5198Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-7.

    Western Blot:

    To detect mIL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    -20 °C

    储存方法

    IL-7 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    IL-7 (IL7) (Interleukin 7 (IL7))

    别名

    IL-7

    背景

    Iterleukin 7 is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL-7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL-7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL-7 stimulates the proliferation of CD4+/CD8+ cells.

    基因ID

    16196

    UniProt

    P10168

    途径

    JAK/STAT Signaling
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