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TRAIL 抗体

TNFSF10 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN5689123
发货至: 中国
  • 抗原 See all TRAIL (TNFSF10) 抗体
    TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
    适用
    • 100
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    宿主
    • 77
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    克隆类型
    • 80
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    多克隆
    标记
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    This TRAIL antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Neutralization (Neut)
    纯化方法
    Anti-hTRAIL/Apo2L specific antibody was purified by affinity chromatography employing immobilized hTRAIL/Apo2L matrix.
    免疫原
    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTRAIL/Apo2L (humanTRAIL/Apo2L).
    Top Product
    Discover our top product TNFSF10 Primary Antibody
  • 应用备注
    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of hTRAIL/Apo2L (30 ng/mL), a concentration of 0.5 - 0.8 μ,g/mL of this antibody is required.

    ELISA:

    To detect hTRAIL/Apo2L by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTRAIL/Apo2L.

    Sandwich:

    To detect hTRAIL/Apo2L by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TRAIL/Apo2L (XP-5289Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTRAIL/Apo2L.

    Western Blot:

    To detect hTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTRAIL/Apo2L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
    限制
    仅限研究用
  • 状态
    Lyophilized
    储存条件
    -20 °C
    储存方法
    TRAIL/Apo2L antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原
    TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
    别名
    TRAIL (TNFSF10 产品)
    别名
    APO2L antibody, Apo-2L antibody, CD253 antibody, TL2 antibody, TRAIL antibody, A330042I21Rik antibody, AI448571 antibody, APO-2L antibody, Ly81 antibody, Trail antibody, tl2 antibody, apo2l antibody, cd253 antibody, trail antibody, apo-2l antibody, trail1 antibody, xtrail1 antibody, tnfsf10l2 antibody, zgc:92320 antibody, TNLG6A antibody, TNF superfamily member 10 antibody, tumor necrosis factor (ligand) superfamily, member 10 antibody, tumor necrosis factor superfamily member 10 antibody, TNF superfamily member 10 S homeolog antibody, TNFSF10 antibody, Tnfsf10 antibody, tnfsf10.S antibody, tnfsf10 antibody
    背景
    Apoptosis or programmed cell death is induced in cells by a group of death domain containing receptors. Binding of ligand to these receptors sends signals that activate members of the caspase family of proteases. The signals ultimately cause degradation of chromosomal DNA by activating DNase. TRAIL (TNF related apoptosis induced ligand) or Apo 2L initiates apoptosis of tumor cells by binding to either of its receptors, DR4 or DR5. These receptors consist of an extracellular TRAIL binding domain and a cytoplasmic "death domain".
    基因ID
    8743
    UniProt
    P50591
    途径
    Apoptosis, Positive Regulation of Endopeptidase Activity
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