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CXCL12 抗体

This anti-CXCL12 antibody is a 兔 多克隆 antibody detecting CXCL12 in WB, ELISA 和 Neut. Suitable for 人.
产品编号 ABIN5689108
发货至: 中国

Quick Overview for CXCL12 抗体 (ABIN5689108)

抗原

See all CXCL12 抗体
CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

适用

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宿主

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克隆类型

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多克隆

标记

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This CXCL12 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 纯化方法

    Anti-hSDF-1-alpha specific antibody was purified by affinity chromatography employing immobilized hSDF-1-alpha matrix.

    免疫原

    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hSDF-1-alpha (human Stromal Cell-Derived Factor-1-alpha).
  • 应用备注

    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of hSDF1α, (100 ng/mL), a concentration of 2-4 μ,g/mL of this antibody is required.

    ELISA:

    To detect hSDF-1-alpha by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hSDF-1-alpha.

    Sandwich:

    To detect hSDF-1α, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human SDF-1α, (XP-5269Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSDF-1α,.

    Western Blot:
    To detect hSDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    -20 °C

    储存方法

    SDF-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

    别名

    SDF-1a

    基因ID

    6387

    UniProt

    P48061

    途径

    Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling
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