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M-CSF/CSF1 抗体

CSF1 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN5689065
发货至: 中国
  • 抗原 See all M-CSF/CSF1 (CSF1) 抗体
    M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
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    多克隆
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    This M-CSF/CSF1 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Neutralization (Neut)
    纯化方法
    Anti-hM-CSF specific antibody was purified by affinity chromatography employing immobilized hM-CSF matrix.
    免疫原
    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hM-CSF (human M-CSF).
    Top Product
    Discover our top product CSF1 Primary Antibody
  • 应用备注
    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of hM-CSF (7.0 ng/mL), a concentration of 0.02 0.03 μ,g/mL of this antibody is required.

    ELISA:

    To detect hM-CSF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hM-CSF.

    Sandwich:

    To detect hM-CSF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human M-CSF (XP-5227Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hM-CSF.

    Western Blot:
    To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
    限制
    仅限研究用
  • 状态
    Lyophilized
    储存条件
    -20 °C
    储存方法
    M-CSF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原
    M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
    别名
    M-CSF (CSF1 产品)
    别名
    CSF-1 antibody, MCSF antibody, C87615 antibody, Csfm antibody, op antibody, csf1-1 antibody, zgc:172186 antibody, CSF1 antibody, csf1-2 antibody, zgc:158436 antibody, colony stimulating factor 1 antibody, colony stimulating factor 1 (macrophage) antibody, colony stimulating factor 1a (macrophage) antibody, macrophage colony-stimulating factor 1 antibody, macrophage colony stimulating factor antibody, colony stimulating factor 1b (macrophage) antibody, CSF1 antibody, Csf1 antibody, csf1a antibody, LOC396599 antibody, LOC100860895 antibody, csf1b antibody
    背景
    Macrophage CSF, also known as CSF1, is produced by monocytes, fibroblasts and endothelial cells. It stimulates the formation of macrophage colonies, enhances antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption by osetoclasts. Natural human MCSF is a dimeric glycoprotein of 70-90 kD molecular weight, existing in multiple glycosylation forms. It binds to a 165 kD glycoprotein of the receptor tyrosine kinase subclass III, a family that includes the receptors for platelet derived growth factor (PDGF) and stem cell factor (SCF).
    基因ID
    1435
    UniProt
    P09603
    途径
    RTK signaling
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