FLT3LG 抗体
Quick Overview for FLT3LG 抗体 (ABIN5688996)
抗原
See all FLT3LG 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Anti-hflt3-Ligand specific antibody was purified by affinity chromatography employing immobilized hflt3-Ligand matrix.
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免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hflt3-Ligand.
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anti-Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-189) antibody
FLT3LG 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-136) antibodyFLT3LG 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 4C4 unconjugated
anti-Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 61-160) antibodyFLT3LG 适用: 人, 小鼠 WB, ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 83-182) antibodyFLT3LG 适用: 人 WB 宿主: 小鼠 Monoclonal D13 unconjugated
anti-Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-184) antibodyFLT3LG 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 1-235) antibodyFLT3LG 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (C-Term) antibodyFLT3LG 适用: 人, 大鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (AA 27-181) antibodyFLT3LG 适用: 人 WB, IF 宿主: 小鼠 Monoclonal 8D10 unconjugated
anti-Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) (C-Term) antibodyFLT3LG 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Fms-Related tyrosine Kinase 3 Ligand (FLT3LG) antibodyFLT3LG 适用: 人 FACS 宿主: 兔 Chimeric unconjugated Fc fragment
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hflt3- Ligand (15 ng/mL), a concentration of 0.25 - 0.75 μ,g/mL of this antibody is required.
ELISA:
To detect hflt3-Ligand by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hflt 3-Ligand.
Sandwich: To detect hFGF-basic by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human FGF-basic (XP-5138Bt) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-basic.
Western Blot:
To detect hflt3-Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hflt3-Ligand is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- flt3-Ligand antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))
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别名
- flt3-Ligand
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背景
- Flt3-Ligand is a growth factor important for the proliferation of hematopoietic cells. Flt3-Ligand binds to, and transmits signals through, the receptor tyrosine kinase known as FMS-like Tyrosine Kinase-3 (FLT-3). Flt3-Ligand promotes long-term expansion and differentiation of human pro-B-cells in the presence of IL-7 or in combination of IL-7 and IL-3. Human Flt3-Ligand can stimulate the proliferation of cells expressing murine FLT-3 receptors.
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基因ID
- 2323
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UniProt
- P49771
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途径
- RTK signaling
抗原
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