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FLT3LG 抗体 (Biotin)

This anti-FLT3LG antibody is a 兔 多克隆 antibody detecting FLT3LG in WB, ELISA 和 Neut. Suitable for 人.
产品编号 ABIN5688995
发货至: 中国
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北京 101111
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Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
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Quick Overview for FLT3LG 抗体 (Biotin) (ABIN5688995)

抗原

See all FLT3LG 抗体
FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

适用

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宿主

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克隆类型

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多克隆

标记

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This FLT3LG antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 纯化方法

    Anti-hFlt3-Ligand specific antibody was purified by affinity chromatography and then biotinylated.

    免疫原

    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFlt3-Ligand.
  • 应用备注

    Neutralization:

    To yield one-half maximal inhibition [ND50] of the biological activity of hFlt-3 Ligand (1.0 ng/mL), a concentration of 4.0-6.0 ng/mL of this antibody is required.ELISA:

    To detect hFlt3-Ligand by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hFlt3-Ligand.

    Sandwich: To detect hFlt-3 Ligand by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Flt-3 Ligand (XP-5138Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFlt-3 Ligand.

    Western Blot:

    To detect hFlt3-Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFlt3-Ligand is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    -20 °C

    储存方法

    flt3-Ligand antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

    别名

    flt3-Ligand

    背景

    Flt3-Ligand is a growth factor important for the proliferation of hematopoietic cells. Flt3-Ligand binds to, and transmits signals through, the receptor tyrosine kinase known as FMS-like Tyrosine Kinase-3 (FLT-3). Flt3-Ligand promotes long-term expansion and differentiation of human pro-B-cells in the presence of IL-7 or in combination of IL-7 and IL-3. Human Flt3-Ligand can stimulate the proliferation of cells expressing murine FLT-3 receptors.

    基因ID

    2323

    UniProt

    P49771

    途径

    RTK signaling
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