CAMK2A 抗体 (pThr286)
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北京 101111
Quick Overview for CAMK2A 抗体 (pThr286) (ABIN5688927)
抗原
See all CAMK2A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr286
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特异性
- CaM Kinase II antibody is specific for a 50 kDa and b 60 kDa CaM Kinase II phosphorylated at Thr286.
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纯化方法
- Affinity Purified
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免疫原
- CaM Kinase II (Thr286) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr286 found in rat brain CaM Kinase II.
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anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 1-478) antibody
CAMK2A 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 305-410) antibodyCAMK2A 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C4 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 305-410) antibodyCAMK2A 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C12 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 305-410) antibodyCAMK2A 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 321-478) antibodyCAMK2A 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) antibodyMicroarray verified CAMK2A 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB443776 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr305) antibodyCAMK2A 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 321-478) antibodyCAMK2A 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) antibodyCAMK2A 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB1880 unconjugated Recombinant Antibody
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr305) antibodyCAMK2A 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Western blots of rat brain lysates with phospho- and dephosphopeptide competition were used to establish the phospho-specificity of the antibody. Applications include Dot Blot (DB) and Western Blot (WB). Dot Blots and Western Blots performed with each lot and working dilutions internally tested under ideal conditions for DB and WB were 1:1000. Suitability for Immunohistochemistry (IHC) has not been determined. Human, mouse, rat and Xenopus have 100 % amino acid sequence identity with the antigen used to raise the antibody.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 100 μLin 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.
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储存条件
- -20 °C
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储存方法
- For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
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- CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))
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别名
- CaM Kinase II
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背景
- Calcium/calmodulin kinase II (CaM Kinase II) is multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to second messengers. Phosphorylation of Thr286 produces an autonomously active form of CaM Kinase II. The antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr286 found in rat brain CaM Kinase II.
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分子量
- 50/60 kDa
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基因ID
- 25400
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UniProt
- P11275
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途径
- WNT signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction
抗原
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