IL-10 抗体
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北京 101111
Quick Overview for IL-10 抗体 (ABIN5688729)
抗原
See all IL-10 (IL10) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- This IgG2A antibody was purified from cell culture by Protein G affinity chromatography.
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免疫原
- E.coli derived Recombinant Murine IL-10
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anti-Interleukin 10 (IL10) (AA 101-178) antibody
IL10 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 19-178) antibodyIL10 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) antibodyIL10 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, 马, 犬, 猴, 山羊, 小鸡, 绵羊, Hamster, Avian, Cat, 驴, 豚鼠 WB 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 10 (IL10) antibodyMicroarray verified IL10 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB971550 unconjugated
anti-Interleukin 10 (IL10) antibodyMicroarray verified IL10 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB312926 unconjugated
anti-Interleukin 10 (IL10) (AA 27-53) antibodyIL10 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 1-178) antibodyIL10 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 1-178) antibodyIL10 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Interleukin 10 (IL10) (AA 21-181) antibodyIL10 适用: 犬 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 19-178) antibodyIL10 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
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应用备注
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ELISA:
Sandwich:
In a sandwich ELISA (assuming 100μ,L/well), a concentration of 4.0-8.0 μ,g/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with ProSci's Polyclonal biotinylated antigen affinity purified anti-murine IL-10 as the detection antibody at a concentration of approximately 0.5-1.0 μ,g/mL.
Western Blot:
To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (0.5 ng/mL), a concentration of 3.0-5.0 μ,g/mL of this antibody is required. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- IL-10 (IL10) (Interleukin 10 (IL10))
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别名
- IL-10
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基因ID
- 3586
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NCBI登录号
- NP_000563
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UniProt
- P22301
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
抗原
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