TRAIL 抗体
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北京 101111
Quick Overview for TRAIL 抗体 (ABIN5688718)
抗原
See all TRAIL (TNFSF10) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- This IgG1k antibody was purified from ascites fluid on a Protein A affinity column.
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免疫原
- The monoclonal antibody was produced in Balb/c X ICR F1 mice using recombinant hTRAIL/APO-II as the immunizing antigen.
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTRAIL/Apo2L (10.0 ng/mL), a concentration of 0.30-0.60 μ,g/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 2.0-4.0 μ,g/mL of this antibody will detect at least 500 pg/mL of recombinant human TRAIL/APO-II when used with our biotinylated antigen affinity purified anti-human TRAIL/APO-II (XP-5289Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μ,g/mL.
Western Blot:
To detect hTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTRAIL/Apo2L is 0.25-1.0 ng/lane, under reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- 4 °C,-20 °C
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储存方法
- Trail/Apo2L lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
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- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
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别名
- TRAIL
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基因ID
- 8743
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UniProt
- P50591
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途径
- Apoptosis, Positive Regulation of Endopeptidase Activity
抗原
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