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VEGF 抗体

This anti-VEGF antibody is a 小鼠 单克隆 antibody detecting VEGF in WB, ELISA 和 Neut. Suitable for 人.
产品编号 ABIN5688712
发货至: 中国

Quick Overview for VEGF 抗体 (ABIN5688712)

抗原

See all VEGF 抗体
VEGF (Vascular Endothelial Growth Factor (VEGF))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This VEGF antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)

克隆位点

4B2-8
  • 纯化方法

    This IgG1k antibody was purified from ascites fluid followed by Protein A affinity chromatography.

    免疫原

    The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant human VEGF as the immunizing antigen.
  • 应用备注

    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10.0 ng/mL), a concentration of <0.2 μ,g/mL of this antibody is required.

    ELISA:

    In a sandwich ELISA (assuming 100uL/well), a concentration of 2.0-4.0 μ,g/mL of this antibody will detect at least 100 pg/mL of recombinant human VEGF165 when used with our biotinylated antigen affinity purified anti-human VEGF165 (XP-5291Bt) as the detection antibody at a concentration of approximately 0.25-1.0 μ,g/mL.

    Western Blot:

    To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    4 °C,-20 °C

    储存方法

    VEGF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
  • 抗原

    VEGF (Vascular Endothelial Growth Factor (VEGF))

    别名

    VEGF

    基因ID

    7422

    UniProt

    P15692
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