VEGF 抗体
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北京 101111
Quick Overview for VEGF 抗体 (ABIN5688712)
抗原
See all VEGF 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- This IgG1k antibody was purified from ascites fluid followed by Protein A affinity chromatography.
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免疫原
- The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant human VEGF as the immunizing antigen.
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10.0 ng/mL), a concentration of <0.2 μ,g/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100uL/well), a concentration of 2.0-4.0 μ,g/mL of this antibody will detect at least 100 pg/mL of recombinant human VEGF165 when used with our biotinylated antigen affinity purified anti-human VEGF165 (XP-5291Bt) as the detection antibody at a concentration of approximately 0.25-1.0 μ,g/mL.
Western Blot:
To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- 4 °C,-20 °C
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储存方法
- VEGF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
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- VEGF (Vascular Endothelial Growth Factor (VEGF))
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别名
- VEGF
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基因ID
- 7422
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UniProt
- P15692
抗原
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