IL-7 抗体
Quick Overview for IL-7 抗体 (ABIN5688700)
抗原
See all IL-7 (IL7) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- This IgG1 antibody was purified from ascites fluid on a Protein A affinity column.
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免疫原
- The monoclonal antibody was produced in BALB/c x C57BL/6 F1 mice using recombinant hIL-7 as the immunizing antigen.
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anti-Interleukin 7 (IL7) (AA 1-177) antibody
IL7 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) antibodyIL7 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) (AA 26-177) antibodyIL7 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) (AA 26-177) antibodyIL7 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) (AA 51-154) antibodyIL7 适用: 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) (AA 26-177) antibodyIL7 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C12 unconjugated
anti-Interleukin 7 (IL7) antibodyIL7 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) (C-Term) antibodyIL7 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 (IL7) antibody (Biotin)IL7 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
anti-Interleukin 7 (IL7) antibody (Biotin)IL7 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL 7 (0.5 ng/mL), a concentration of 0.05-0.08 μ,g/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 2.0-4.0 μ,g/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-7 when used with our biotinylated antigen affinity purified anti-human IL-7 (XP-5197Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μ,g/mL.
Western Blot:
To detect hIL-7 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-7 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- 4 °C,-20 °C
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储存方法
- IL-7 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
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- IL-7 (IL7) (Interleukin 7 (IL7))
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别名
- IL-7
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背景
- Iterleukin 7 is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL-7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL-7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL-7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL-7 is not affected by antibodies to the T cell growth factors such as IL-2, IL-4 and IL-6, suggesting that IL-7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL-7 and Con A, but not to IL-7 alone. In myeloid lineage cells, IL-7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues.
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基因ID
- 3574
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UniProt
- P13232
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途径
- JAK/STAT Signaling
抗原
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