CCL3 抗体
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北京 101111
Quick Overview for CCL3 抗体 (ABIN5688699)
抗原
See all CCL3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- This IgG1 antibody was purified from ascites fluid by SAS precipitation followed by ion exchange column.
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免疫原
- The monoclonal antibody was produced in C3H/HeJ mice using recombinant hMIP-1α, as the immunizing antigen.
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anti-Chemokine (C-C Motif) Ligand 3 (CCL3) antibody
CCL3 适用: 大鼠, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) antibodyCCL3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) antibodyCCL3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4E7 unconjugated
anti-Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 25-81) antibodyCCL3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) antibodyCCL3 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) antibodyCCL3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 59-87), (C-Term) antibodyCCL3 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB21614 unconjugated
anti-Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) antibodyCCL3 适用: 大鼠 ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) antibodyCCL3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) antibodyCCL3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1α, (100 ng/mL), a concentration of 5.0-10.0 μ,g/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 2.0-4.0 μ,g/mL of this antibody will detect at least 2000 pg/mL of recombinant human MIP-1α, when used with our biotinylated antigen affinity purified anti-human MIP-1α, (XP-5232Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μ,g/mL.
Western Blot:
To detect hMIP-1α, by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1α, is 1.0-2.0 ng/lane, under non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- 4 °C,-20 °C
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储存方法
- MIP-1α, lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
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- CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
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别名
- MIP-1a
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背景
- Macrophage Inflammatory Protein 1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77 % and 70 % amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.
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基因ID
- 6348
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UniProt
- P10147
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途径
- Cellular Response to Molecule of Bacterial Origin, Autophagy
抗原
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