GZMB 抗体 (AA 7-227)
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北京 101111
Quick Overview for GZMB 抗体 (AA 7-227) (ABIN5688512)
抗原
See all GZMB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 7-227
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纯化方法
- Granzyme B (monoclonal B18.1) antibody was purified to ≥,95% in SDS-PAGE.
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免疫原
- Granzyme B (monoclonal B18.1) antibody was raised against recombinant human granzyme B, amino acids 7-227.
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亚型
- IgG2a
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anti-Granzyme B (GZMB) (AA 21-247) antibody
GZMB 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Granzyme B (GZMB) (AA 73-187) antibodyGZMB 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal GZMB-3014 unconjugated
anti-Granzyme B (GZMB) (AA 21-130) antibodyGZMB 适用: 人, 小鼠 WB, ELISA, FACS, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Granzyme B (GZMB) antibodyGZMB 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Granzyme B (GZMB) (AA 1-247) antibodyGZMB 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Granzyme B (GZMB) (AA 73-187) antibodyGZMB 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal GZMB-3056 unconjugated
anti-Granzyme B (GZMB) antibodyGZMB 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal CLB-GB11 unconjugated
anti-Granzyme B (GZMB) (AA 21-247) antibodyGZMB 适用: 人 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 4E4 unconjugated
anti-Granzyme B (GZMB) (AA 21-247) antibodyGZMB 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2G12G7 unconjugated
anti-Granzyme B (GZMB) antibodyGZMB 适用: 人 ELISA, FACS, cELISA 宿主: 小鼠 Monoclonal GM-4C1 unconjugated
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应用备注
- Granzyme B antibody can be used for detection of human granzyme B by flow cytometry, immunocytochemistry, immunohistochemistry (frozen sections, paraffin sections), immunoprecipitation and western blot. Method: Permeabilized human T cells (5 x 105) were incubated on ice for 30 min in 50 μ,l FACS buffer (PBS, 5 % Fetal calf serum, 0.02 % azide) containing 1 μ,g of FITC- labeled B18.1 antibody. After washing in FACS buffer, cells were analyzed by flow cytometry. Method: Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1 % BSA, slides were incubated for 1 hr at room temperature (RT) with 20 μ,g/mL of B18.4 antibody in PBS, 0.1 % BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope. Method: : Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1 μ,g/mL. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS containing 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Granzyme B antibody can be stored at 4°C, stable for one year.
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- GZMB (Granzyme B (GZMB))
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别名
- Granzyme B
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基因ID
- 3002
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UniProt
- P10144
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途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
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