Neurotrophin 3 抗体
Quick Overview for Neurotrophin 3 抗体 (ABIN5687601)
抗原
See all Neurotrophin 3 (NTF3) 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Anti-hNT 3 specific antibody was purified by affinity chromatography employing immobilized hNT-3 matrix.
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免疫原
- Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hNT-3 (human Neurotrophin-3).
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hNT-3 (50.00 ng/mL), a concentration of 2.0 - 4.0 μ,g/mL of this antibody is required.
ELISA:
To detect hNT-3 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hNT-3.
Sandwich:
To detect hNT-3 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human NT-3 (XP-5249Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNT-3.
Western Blot:
To detect hNT-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNT-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- NT-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- Neurotrophin 3 (NTF3)
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别名
- NT-3
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基因ID
- 4908
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UniProt
- P20783
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途径
- RTK signaling, Neurotrophin Signaling Pathway
抗原
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