In Immunoelectrophoresis against Human serum a single precipitin line is obtained. The antiserum does not react with any other protein component of Human serum or plasma. The antiserum does not cross-react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Hyperimmune antisera with strong precipitating activity are selected for fractionation by Salt Precipitation and purification of the IgG fraction by DEAE-Chromatography
免疫原
The antigen has been isolated from pooled Human plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Liquid
溶解方式
Restore by adding 1 mL of sterile distilled water
浓度
8.0 mg/mL
缓冲液
PBS, pH 7.2 without preservatives
储存液
Without preservative
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原
CFP
(Complement Factor P (CFP))
别名
Properdin
背景
Properdin is a factor of the alternative complement activation pathway. It has a molecular weight of 222,000 and consists of four peptide chains. Properdin is stabilizing the C3 convertase C3bBb. The mean concentration in plasma is 20 mg/mL.Synonyms: CFP, Complement factor P, PFC