C5 抗体 (FITC)
Quick Overview for C5 抗体 (FITC) (ABIN568577)
抗原
See all C5 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The reactivity of the antiserum is restricted to C5. In immunoelectrophoresis and Radial Immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C5 protein. No reaction is obtained with any other plasma protein component or serum. The antiserum does not cross react with any other component of human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
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产品特性
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Absorption / Emission: 492 nm / 515 nm
Molar Ratio: Fluorescein/IgG ~1.4 -
纯化方法
- The IgG fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by Immunoelectrophoresis and Double Radial Immunodiffusion.
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免疫原
- C5 protein is isolated as a homogenous protein for use in antiserum production. Freund’s complete adjuvant is used in the first step of the immunization
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亚型
- IgG
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anti-Complement Component 5 (C5) (AA 1-18), (N-Term) antibody
C5 适用: 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 5 (C5) (AA 701-755) antibodyC5 适用: 人, 小鼠 WB, ELISA, IF (p), IF (cc), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 5 (C5) (AA 253-281) antibodyC5 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 5 (C5) (AA 253-281), (N-Term) antibodyC5 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB21866 unconjugated
anti-Complement Component 5 (C5) (C-Term) antibodyC5 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 5 (C5) (AA 1-77) antibodyC5 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 5 (C5) (AA 253-281), (N-Term) antibodyC5 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Complement Component 5 (C5) (AA 1550-1661) antibodyC5 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore by adding 1 mL of sterile distilled water.
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浓度
- 10 mg/mL
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid Repeated thawing and freezing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- C5 (Complement Component 5 (C5))
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别名
- Complement C5
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背景
- C5 component of human complement is a plasma protein consisting of two polypeptide chains, alpha, beta, bound by disulphide bonds. It has a molecular weight of 190,000. The activated protein forms with the complement factors C6-C9 the membrane attack complex.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, CPAMD4, Complement component 5
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基因ID
- 727
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NCBI登录号
- NP_001726
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UniProt
- P01031
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途径
- Complement System, Carbohydrate Homeostasis
抗原
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